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Fig. 3 | Cell & Bioscience

Fig. 3

From: KLF7/VPS35 axis contributes to hepatocellular carcinoma progression through CCDC85C-activated β-catenin pathway

Fig. 3

Overexpression of KLF7 aggravates cell progression in HCC cell lines. a Overexpression efficiency of KLF7 in Huh-7 and SHEP1 cells was confirmed by western blot. b, c Cell viability of KLF7-overexpressing Huh-7 and SHEP1 cells was determined by CCK-8 assay. d, e Colony formation of KLF7-overexpressing Huh-7 (p < 0.001) and SHEP1 (p < 0.01) cells were detected by colony formation assay. f Flow cytometry was preformed to detect the progression stages of cell cycle in KLF7-overexpressing Huh-7 and SHEP1 cells. g, h Statistical results of cell cycle in Huh-7 (p < 0.01) and SHEP1 cells (p < 0.01). i, j Cell apoptosis was determined after the transfection of pcDNA3.1-KLF7 or empty vector into Huh-7 and SHEP1 cells. k Representative images of transwell assay. l, m Transwell invasion assay of Huh-7 (p < 0.01) and SHEP1 (p < 0.001) cells transfected with KLF7-overexpressing vectors or empty vectors. *p < 0.05, **p < 0.01, ***P < 0.001

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Cell & Bioscience

ISSN: 2045-3701

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