Fig. 4

The Mbp/L1-70 axis drives myelin-induced NPC dendrite regeneration via PPARγ. a Nuclear fractions from wild-type (WT) and Mbp-null (Mbp^−/−) spinal cord-derived E12 mouse neural progenitor cells (E12 mNPCs) transduced with pcDNA3-Ctrl or pcDNA3-L1-70 were applied to substrate-coated recombinant PPARγ for ELISA. Binding of nuclear L1-70 to recombinant PPARγ was assayed using L1cam antibody-172-R. b–m Analysis of neuritogenesis in b–e WT or Mbp^−/− E12 mNPCs, f–i WT or Mbp^−/− spinal cord-derived E14 rat neural progenitor cells (E14 rNPCs), and j–m D1 WT or Mbp^−/− NPCs derived from human iPSCs (D1 hNPCs) transduced with pcDNA3-Ctrl or pcDNA3-VP16-PPARγ2 and treated with the PPARγ agonist ciglitazone (1 µM) after 48 h of Poly-d-lysine control (PDL Ctrl) or myelin (Mye) substrate culture. WT PDL Ctrl was used to normalize values for mNPCs and rNPCs. WT Lam Ctrl was used to normalize values for hNPCs. All panels report means ± standard deviations (SDs). n = 3 embryos/genotype × 3 wells/embryo. *P < 0.05, **P < 0.01 [two-way ANOVA, post-hoc Tukey’s test]