Fig. 1

Identification of BMMs in vitro and LPS stimulation upregulates AR expression in BMMs. a Following induction of BMMs from the bone marrow, approximately 98% of the cells were F4/80-positive. b Confocal microscopy of BMMs from WT mice left untreated or treated for 24 h with LPS (500 ng/ml), and then immunostained for AR. Scale bars = 10 µm. Right graph shows quantification analysis of the number of AR + foci per cell. c, d mRNA and protein levels of AR in BMMs from WT mice left untreated or treated with 500 ng/ml LPS for 24 h. Transcription levels are expressed as fold change of control and densitometric AR/β-actin ratios are shown below the blot. Data are expressed as the mean ± SD from three independent experiments, *P < 0.05, **P < 0.01