Fig. 6

ATRi and HSP90i share common molecular targets. A673 cells were treated with 45 nM AUY922 ± 2 µM VE821 for 24 h and a quantitative whole proteome analysis was done by mass spectrometry from three individual experiments (refer to Additional file 11: File S1). The proteome data set was further processed by ingenuity pathway analysis (IPA; cutoff: q < 0.05) for alterations among canonical pathways (a), diseases and bio functions (c) and upstream regulators (d). The activation z-score of individual processes/molecules is shown in yellow for activation and blue for inactivation. b Analysis of indicated proteins was done by Western blot; vinculin was used to control protein loading. Immunoblots are representative of at least two independent experiments. e IPA of toxicities after AUY922 in combination with VE821 (AUY-VE). f DEPs of individual treatments (cutoff: q < 0.05) and their overlaps