Genotyping of Agmo transgenic mice by qPCR and by conventional PCR. a Conventional genotyping of Agmo-lacZ cohorts (wt/wt: 476 bp, tg/wt: 476 bp + 1090 bp). b LacZ qPCR to count allele copy numbers of the Agmo-lacZ transgene and c Agmo-WT qPCR to determine copy numbers of the native Agmo allele. Red arrows indicate homozygous Agmo-lacZ animals determined by lacZ qPCR. Y-axes were cut off at ∆CT 5. Data shown as mean ± S.D.