Fig. 5
ApoE4 (Δ272–299) promotes ER-mitochondria interaction and mitochondrial Ca2+ overload. a TEM analysis of the MAM in the hippocampus of wild-type and apoE4 (Δ272–299) transgenic mice. b Fluorescent microscopy analysis of MAM in apoE4 (Δ272–299)-expressed N2a cells. The scale bar is 10 µm. c Longitudinal analysis of Ca2+ levels in mitochondria of N2a cells following inducing apoE4 (Δ272–299) expression. d Induction of GRP75 over-expression increased the contact areas of MAM in N2a cells. The scale bar is 10 µm. e Treatment with MKT077 mitigated the MAM changes induced by apoE4 (Δ272–299) in N2a cells. The scale bar is 10 µm. Data are representative images or expressed as the mean ± SEM of each group (n = 3) from three separate experiments. **P < 0.01, versus the wild-type mice. The significant differences were determined by ANOVA and post hoc Fisher’s least significant difference