Fig. 1

ApoE4 (Δ272–299) triggers ER stress in vivo and in vitro. a Western blot analysis the relative levels of CHOP, GRP78 and GRP75 expression in the hippocampus of apoE4 (Δ272–299) transgenic and the age- and sex-matched wild-type mice. b Transfection with pEGFP-apoE4 (Δ272–299) induces EGFP expression in N2a cells. c Western blot analysis of the relative levels of CHOP, GRP78 and GRP75 expression in the different groups of N2a cells. d Treatment with PBA mitigated the apoE4 (Δ272–299)-up-regulated CHOP, GRP78 and GRP75 expression in N2a cells (^ΔΔP < 0.01 versus the wild-type mice; **P < 0.01 versus the N2a cells transfected with pIRES vector; ^##P < 0.01 versus the apoE4 (Δ272–299)-expressed cells). Data are representative images or expressed as the mean ± SEM of each group (n = 3) from three separate experiments. The significant differences were determined by ANOVA and post hoc Fisher’s least significant difference