Fig. 2
EGFL6 promotes CRC cell proliferation. a, b Relative EGFL6 mRNA level and protein expression of FHC (human normal colon epithelial cell), HCT116 (human colorectal carcinoma epithelial cell) and HT29 (human colorectal epithelial adenocarcinoma cell). c EGFL6 expression of HCT116 culture medium presenting in western blot. CM: condition medium. d The relative viability of HCT116 (2 \(\times\) 103 cell/well) and HT29 (3 \(\times\) 103 cell/well) treated from various concentrations of human recombinant EGFL6 (0.1, 1 or 10 ng/mL), incubated 5 days in 96 well for SRB. e Cell proliferation signals (p-ERK (extracellular signal–regulated kinase), p-AKT (protein kinase) protein expression after EGFL6 (10 ng/mL) treatment by different time point. f EGFL6 mRNA expression of HCT-116 and HT29 after knockdown EGFL6. Incubated 48 h after siRNA treatment. g, h Tumor cell viability as well as ERK and AKT phosphorylation of HCT-116 and HT29 after EGFL6 knockdown. Incubated 48 h after siRNA treatment. *p < 0.05, **p < 0.01, ***p < 0.005