Fig. 4

EGR2 expression is reduced in asthma. a the heatmap of DEGs related to asthma in respiratory epithelial cells, obtained from the GSE64913 dataset; the right upper histogram represents color gradation; b the Venn diagram showing the DEGs in respiratory epithelial cells from the GSE64913 dataset, downstream genes of miR-182 from the miRDIP database, and human transcription factors from the Cistrome database; c EGR2 mRNA expression in human PBMCs determined by RT-qPCR; d Western blot analysis of EGR protein in human PBMCs; e EGR2 mRNA expression in mouse spleen cells detected by RT-qPCR; f Western blot analysis of EGR2 protein in mouse spleen cells; Comparisons between two groups were conducted by unpaired t test. * p < 0.05, compared with the normal individuals (normal), normal mice (normal-M); n = 22 in normal individuals; n = 29 in asthma patients; n = 12 in normal mice; n = 12 in asthma mice. Each experiment was repeated 3 times independently