Fig. 9
From: Identification of a potent antagonist of smoothened in hedgehog signaling
0025A inhibits Hh-dependent hair growth and hair follicle morphogenesis after depilation. a Eight-week old female C57BL/6 mice were shaved. Then the bottom half of dorsal region were depilated with Nair and treated once daily with 30 μL of vehicle control (95% acetone/5% DMSO) or 5 mM Smo antagonist 0025A for 14 consecutive days. The photos were taken on 0 Day, 10 Days and 14 Days after depilation. The dotted boxes represented the depilated area. b Hematoxylin and eosin staining on dorsal skin sections (vehicle and 0025A, n = 3). Scale bar, 10 µm