Fig. 3

High NEAT1 expression in EVs promotes BC cell invasion and migration. a Laser scanning confocal microscopy was applied to observe the uptake of EVs by BC cells; b–d. MTT assay was applied to detect the effect of EVs on BC cell proliferation; e NEAT1 expression in MCF-7 cells before and after serum EV treatment detected by RT-qPCR; f–h Transwell assay was utilized to detect the effect of EVs on BC cell invasion; i–k Scratch test was used to detect effect of EVs on BC cell migration; l Lung metastases in nude mice were used to detect the effect of EVs on BC cell metastasis in vivo. n = 5; Three independent experiments were performed; control group is the remaining supernatant after EVs extraction; data in panel e were tested by t test, data in panels b, c, d, f, g, h, i, j and k were analyzed using two-way ANOVA, and data in panel l were analyzed using one-way ANOVA. Tukey’s multiple comparisons test was used for pairwise comparisons after ANOVA; * compared to the control group or 0 µg/mL, p < 0.05