Fig. 1

Screening the hub genes of HCC. a Heatmap shows the up- and down-regulated genes between tumor and normal samples in different datasets. Genes (row) are clustered to two groups for better visualization and patient samples (column) are ordered according to tumor/normal types. Color scheme is based on z-score distribution, from blue to red Genes (rows) with (log2 fold change) > 1 and adjusted P-value (adj.p) < 0.01 are listed in respective of each dataset. b Venn plot of the differentially expressed genes in four datasets. c Significantly enriched terms in the Reactome pathway database (y-axis) are shown in bar plots based on the up-regulated genes in the tumor samples compared with normal samples. d The bubble plot displays the enrichment of GO terms. The y‑axis indicates the significance of the term (− log10 adjusted P‑value) and the x‑axis indicates the Z‑score. Bubbles indicate the GO terms, with green indicating BP terms, red indicating cellular component terms and blue indicating molecular function terms. The bubble size indicates the gene numbers in the GO terms. e Circular plots illustrating the enriched Reactome pathways. The genes are bridged by ribbons to their assigned enriched pathways. The white-to-red color indicates the ranking of the number of enriched Reactome pathways. f Log-rank test P-values of the hub genes of HCC