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Fig. 3 | Cell & Bioscience

Fig. 3

From: Comparative evaluation of methods for isolating small extracellular vesicles derived from pancreatic cancer cells

Fig. 3

SEC purification process of the pellet obtained via ultracentrifugation. a The picture of the ultracentrifuge tube before and after ultracentrifugation. The border of the concentrate and ultracentrifugation rose solution became vague after ultracentrifugation. b Fraction analysis of DG ultracentrifugation (n = 3). Because of high CD63 concentration, the fraction 6, 7, 8, 9 and 10 were merged as the purified sample. c The picture of SEC, the EVs concentrate was eluted. d Fraction analysis of SEC (n = 3). Because of high CD63 concentration, the fraction 9 and 10 were merged as the SEC purified sample. * P < 0.05. Red color indicates the cell culture supernatant

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