Fig. 7

SOX4 transcriptionally activates HDAC1 in multiple types of cancer. a Prediction of SOX4 binding sites at the promoter of HDAC1 and the design of four HDAC1 promoter fragments and five mutated HDAC1 promoters for active binding sites analysis. b The transcriptional activity of HDAC1 promoter in HCT-116 and HT-29 cells was analyzed by luciferase reporter assay. c SOX4 promotes the transcriptional activity of HDAC1 promoter in HCT-116 and HT-29 cells. The luciferase reporter plasmids driven by HDAC1 promoter were transfected into SOX4-overexpressing or control HCT-116 and HT-29 cells. The luciferase activity in these cells was determined by Dual-luciferase Reporter Assay. d The transcriptional activities of indicated fragments of HDAC1 promoter in SOX4-overexpressing and control HCT-116 and HT-29 cells were analyzed. e The transcriptional activities of indicated mutated HDAC1 promoters in SOX4-overexpressing and control HCT-116 and HT-29 cells were analyzed. f, g SOX4 directly binds to the promoter of HDAC1 in colorectal cancer cells. f ChIP-PCR was employed to determine the interaction between SOX4 protein and HDAC1 promoter. g DNA pull-down assay was performed to determine the interaction between indicated DNA fragments and SOX4 protein. Data are represented as mean ± s.d.; *P<0.05, **P<0.01, ***P<0.001; two-tailed Student’s t-test