Fig. 3
From: Neuregulin 1/ErbB4 signaling contributes to the anti-epileptic effects of the ketogenic diet
KD treatment exhibits little effect on excitatory synaptic transmission in the hippocampus. a Diagram of hippocampal slice recording. Pyramidal neurons in the CA1 region were clamped in whole-cell configuration. Evoked excitatory postsynaptic currents (eEPSCs) were recorded under the stimulation of Schaffer collateral inputs by using a concentric bipolar electrode. b Representative eEPSC traces. Scale bars, 20 ms and 500 pA. c Comparable amplitudes of eEPSCs in the CA1 region of hippocampus between CD and KD group. n = 28 neurons from 4 mice fed CD; n = 24 neurons from 4 mice fed KD. Two-way ANOVA, F(1,450) = 0.05, P = 0.8231. d Representative sEPSC traces. Scale bars, 2 s and 10 pA. e, f Unaltered sEPSC frequency and amplitude between two groups. n = 26 neurons from 4 mice fed with CD; n = 20 neurons from 4 KD-fed mice. Student’s t test, for frequency, t(44) = 0.4743, P = 0.6376;, for amplitude, t(44) = 0.3979, P = 0.6926