Fig. 1

IL-15 expression in the placenta of different trimesters and trophoblast cell lines. a IL-15 mRNA level in human placental samples from different trimester as determined by RT-PCR. First trimester: 1st (n = 3), Second trimester: 2nd (n = 3), and Third trimester: 3rd (n = 3). b, c Relative expression of IL-15 protein in placenta tissues from different trimesters and representative images as measured by western blot. d Immune-fluorescence double staining of IL-15 (green) and CK7 (red) in placental samples of different trimesters. e Immune-fluorescence double staining of IL-15 (green) and vimentin (red) in placental samples of different trimesters. f Immune-fluorescence double staining of IL-15 (green) and CD31 (red) in placental samples of different trimesters. For d-f, bar = 50 μm. g IL-15 mRNA expression in trophoblast cell lines (HTR-8/SVneo, JEG-3, JAR) and HUVEC (n = 3). h, i IL-15 protein expression in trophoblast cell lines and HUVEC and representative images as determined by western blot. *P < 0.05, ** P < 0.01