Fig. 6

Dab2-Ser24 phosphorylation is involved in the regulation of cytoskeletal reorganization associated with outside-in signaling of integrin αIIbβ3. a, b Platelets with the indicated treatments were spread on coverslips. After spreading on coverslips, the platelets were fixed, permeabilized, stained for F-actin (green) and observed by phase contrast microscopy under a high power field (HPF) of 1,000 X magnification. The number of platelet adhesion/HPF and platelet spreading (area > 10 μm²)/HPF in each group is shown. The data are presented as the mean ± SEM of 2 independent experiments with four assays. ns, no significance. c, d After spreading on coverslips, the platelet lysates were collected for Western blotting analysis. The proteolytic cleavage products of Dab2 were marked as *. The level of Dab2-Ser24 phosphorylation in the platelets pretreated with R11 peptide was arbitrarily set as 1. The data are presented as the mean ± SEM of 2 ~ 6 independent experiments. e, f After spreading on coverslips, the platelets were fixed, permeabilized, stained for F-actin (green) and observed by phase contrast microscopy under a high power field (HPF) of 1,000 X magnification. The number of platelet adhesion/HPF and platelet spreading (area > 10 μm²)/HPF in each group is shown. The data are presented as the mean ± SEM of 2 ~ 4 independent experiments. ns, no significance. **p < 0.01; ***p < 0.001