Fig. 5

Tissue differentiation in A375 xenograft tumors. (A and B) RT-qPCR detection of expression of genes for neuronal and neural stemness (a) and genes for mesodermal or/and endodermal tissue differentiation (b) in cells and tumors. Significance of gene expression change was calculated based on experiments in triplicate using two-tailed Student’s t-test. Data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. NS: not significant. c IHC detection of cell/tissue markers in tumor sections. Below the marker panels are corresponding sections stained with HE. Objective magnification for sections: 20×; insets: 40×