Fig. 2

Tumorigenicity and differentiation potential of neural stem cell NE-4C. a Tumor formation by NE-4C in nude mice. b Tissue differentiation in NE-4C xenograft tumors examined by histology. A partial overview of a section with HE staining and particular tissue types in the section are shown. Original objective magnifications for sections: 4× for overview, 40× for specific tissues. c–e Differential expression of genes representing neural stemness (c), neuronal differentiation (d), and mesendodermal tissue differentiation (e) in NE-4C cells and tumor, as detected with RT-qPCR. Significance of expression change was calculated based on experiments in triplicate using two-tailed Student’s t-test. Data are shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. NS: not significant. f IHC detection of cell/tissue markers in tumor sections. Below the marker panels are corresponding sections stained with HE. Objective magnification: 20×; insets: 40×