Fig. 7

PXR inhibited the phosphorylation of JNK. a Phosphorylation of Erk, p38, and JNK detected by flow cytometry in Caco-2 cells treated with LPS for different times and statistical analysis of MFI. b and c Phosphorylation of JNK in Caco-2 cells treated with different concentrations of LPS for 30 min and statistical analysis of MFI. d and e Phosphorylation of JNK in empty or PXR-overexpressing Caco-2 cells treated with LPS and statistical analysis of MFI. f and g Phosphorylation of JNK in Caco-2 cells was detected by Western blotting and statistical analysis of relative expression. h Phospho-JNK in the terminal ileum of mice was detected by immunofluorescence and statistical analysis of average optical density. Data are expressed as mean values ± SD. n = 3 ~ 5 per group, representative data from three independent experiments. Statistical analyses were performed with Student’s two-tailed unpaired t-test, *p < 0.05; **p < 0.01; ***p < 0.001; n.s., not significant; JNK, c-Jun N-terminal kinase; MFI, mean fluorescence intensity. Scale bars represent 20 μm in (h)