Fig. 2

ShRNA-Mettl14 can relieve SCI and promote the recovery of neurites after SCI. ShRNA-Mettl14 was injected into SCI model rats using lumbar puncture via the tail vein. The equal distance transverse section on 2-cm spinal cord centred on the injured site was made. Mettl14 levels in the spinal cord tissue of rats at the 30th day were detected by RT-qPCR (a) and Western blot analysis (b). At the 30th day after SCI, the content of m6A in the total RNA of the spinal cord was measured by EpiQuik m6A RNA methylation quantification kit (c). BBB motor function score (e) and level test score (d) were used to evaluate the motor function of the rats in each group. The rats were euthanized at 30th day, and HE staining was performed (f); the arrow shows the weakened destruction of central gray matter and peripheral white matter; Western blot analysis (g) were used to measure the expression of AcTub and MAP2. The positive expression of GFAP, NeuN and NF-200 was evaluated by immunohistochemical staining; and the arrow indicates GFAP, NeuN and NF-200 positive expression (h). N = 8. Each experiment was repeated three times independently. The data are expressed by mean ± standard deviation. The data in panels A/B/C/G were analysed by one-way ANOVA, and data in panels d–f were analysed by two-way ANOVA, followed by Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01