Fig. 4

Knockout CCM3 compromised mitochondrial membrane potential. a Immunofluorescence images and quantification (b) of mitochondria in selected CRISPR-CCM3 EPCs and s-oeATPIF1 EPCs, scale bar, 50 μm. c mitochondrial fusion and fission markers in selected EPCs. d mitochondrial membrane potential in selected EPCs by JC-1 assay, cells were treated with FCCP (10 μM) for 2 h, DMSO was used as negative control. Mean fluorescence intensity (MFI) was measured by Image J, n = 3, *p < 0.05, **p < 0.01, scale bar, 10 μm