Fig. 4
From: Retinol from hepatic stellate cells via STRA6 induces lipogenesis on hepatocytes during fibrosis
Knock-down of STRA6 inhibits the release of retinol from activated HSCs. a The LX2 cell line was treated with 1 μM retinol for 3 days following transfection with siRNAs targeting human TIF1γ and/or STRA6. The DIC channel (bright image) shows the cell morphology, and retinol autofluorescence is shown in blue. Reproducible result from two independent experiments was shown. Scale bar: 10 μm. b, c The experimental conditions were the same as described for A. Retinol autofluorescence is shown in blue, αSMA is shown in red, and TIF1γ (b) or STRA6 (c) is shown in green. The nuclei were stained with TO-PRO 3 (magenta). Reproducible result from two independent experiments was shown. Retinol or αSMA or TIF1γ-positive area % per 0.02 mm2 were quantified using Image J program. Reproducible result from three independent experiments was shown. **P < 0.01, ***P < 0.001, ****P < 0.0001, ns; non-significant. Scale bar: 10 μm