Fig. 3

KLHL18 regulates non-small cell lung cancer cell proliferation, migration, and invasion through the PI3K/AKT/mTOR pathway. a Spectrometry analysis with NCI-A549 cells revealed that KLHL18 protein can bind to PI3KP85α. b, c The effect of KLHL18 protein expression on the expression of effector proteins and pathway proteins in the b NCI-A549 and c NCI-H1299 cell lines. The lower graph corresponds to the gray value statistical graph, *P < 0.05, **P < 0.01. d, e PD-L1 protein levels decreased after a PI3K pathway inhibitor was added in both NCI-A549 (d) and NCI-H1299 (e) cell lines. The lower graph corresponds to the gray value statistical graph of various protein changes, *P < 0.05, **P < 0.01. f In the qRT-PCR experiments, the mRNA level of PI3KP85α did not change with the expression of KLHL18