Fig. 5

The reduction of Lrp4 expression in hippocampus increased the threshold of seizures. a Schematic diagram of the site of stereotactic injection of lentivirus. The expression of GFP showed that virus was precisely injected into lacunosum-moleculare layer (LMol) and molecules layer (Mol) of the hippocampus in mice. b Lrp4 protein level of the hippocampus in the mice, which were trans-infected by sh control lentivirus (sh pll3.7 vector injection), sh Lrp4 scramble lentivirus, sh Lrp4 lentivirus, Actin as an internal control in Western blotting. c Quantification of Lrp4 protein level in b. Lrp4 protein expression decreased about 70% in sh Lrp4 lentivirus trans-infected mice. Three separate experiments were performed in duplicate (**p < 0.01). d Representative time courses of seizure development by repeated pilocarpine injection. Different virus trans-infected mice were subjected to pilocarpine injection every 30 min and seizure stages were scored at the same time. e Increased number of pilocarpine injections were needed to reach stage 5 seizure for sh Lrp4 lentivirus trans-infected mice (10 injections), comparing with sh control lentivirus (5 injections) and sh Lrp4 scramble lentivirus (5 injections) trans-infected mice. (n = 10 mice for each group; **p < 0.01). f Increased latency of sh Lrp4 lentivirus trans-infected mice to generalized convulsive seizure in response to PTZ. (n = 10 mice for each group; **p < 0.01)