Fig. 1

Seizures induced by pilocarpine decreased Lrp4 expression in mice hippocampus. a Relative mRNA level of Lrp4 in the hippocampus of pilocarpine injected mice, which were collected at different time point (0 h, 2 h, 4 h, 12 h) after injection. Comparing to control mice (vehicle injection), Lrp4 mRNA decreased after injection (2 h, 4 h, 12 h). b X-gal staining the brain slice of pilocarpine injected Lrp4-lacZ/+ mice. β-Gal activity decreased in both stratum lacunosum-moleculare layer (LMol) and molecules layer (Mol) of hippocampus. c Lrp4 protein level in the hippocampus of pilocarpine injected mice, which were collected at different time point (0 h, 2 h, 4 h, 12 h) after injection, Actin as an internal control. d Quantification of Lrp4 protein level in c, Lrp4 protein decreased after injection (4 h, 12 h). For each experiment, three separate experiments were performed in duplicate (**p < 0.01)