Fig. 7
SDF-1α induces different interaction patterns between Gβ1 and GRK depending on the receptor. a Each of the chemokine receptors (CXCR4/CXCR7) was expressed in HEK293 cells together with Gβ1 tagged with SmBit at the N-terminal and GRK2/GRK5 tagged with LgBit at the C terminal. Luciferase activities induced by SDF-1α or not (Veh.) were then measured. b Cells expressing CXCR4/CXCR7 tagged with LgBiT at the C-terminal and Gβ1-SmBiT were treated with SDF-1α or not (Veh.), and then luminescence was measured. c Cells expressing CXCR4/CXCR7 tagged with LgBiT at the C-terminal and GRK2-SmBiT were treated with SDF-1α or not (Veh.), and then luminescence was measured. Results are the average of three independent experiments. Values are presented as the mean ± SD