Fig. 2
P4-dependent induction of Pla2g10 is mediated via its nuclear receptor, PR, during estrous cycle in the uterus. a Analyses of expression levels of Pla2g10 mRNAs in mouse uterus exposed to various concentrations of P4 (0.25–2 mg). b-c Evaluation of the inhibitory action of PR antagonist, RU-486 (1 mg/mouse) on P4 (2 mg/mouse)-induced Pla2g10 expression in uteri of OVX mice (n = 5 per each group) by b RT-PCR and real-time RT-PCR and c immunofluorescence. The bottom panels show higher magnification images of the boxed area. d Analyses of expression levels of Pla2g10 mRNAs during estrous cycle. e Immunofluorescence of PLA2G10 in the uterus during estrous cycle. The bottom panels show higher magnification images of the boxed area. Green and red colors indicate the presence of PLA2G10 and nuclei, respectively. RU RU-486, LE luminal epithelium, GE glandular epithelium, S stroma, Di diestrus, Pro proestrus, Est estrus, Met metestrus. Scale bar: 50 µm. *p < 0.05