Fig. 2

Phloretin (PT) reduced body weight in mice with HFD-induced obesity. a Male mice were fed a HFD (containing 60% fat) for 16 weeks and administered DMSO, 10 mg/kg phloretin (PT10), or 20 mg/kg phloretin (PT20) via intraperitoneal injection (I.P.) twice a week from week 4 to week 16. b Appearance of the mice. c Body weight was measured for 16 weeks. d Body weight was measured in the last week and e weight gain measured from the fourth week to the 16th week. f Food intake was monitored each day. Phloretin (PT) reduced the epididymal and inguinal adipose tissue weight in HFD-induced obese mice. g HE staining of epididymal adipose tissue (200 × magnification). h Weight of epididymal adipose tissue. i Size of adipocytes in epididymal adipose tissue. j HE staining of inguinal adipose tissue (200 × magnification). k Weight of inguinal adipose tissue. l Size of adipocytes in inguinal adipose tissue. Data are presented as mean ± SEM; n = 10. *P < 0.05, **P < 0.01 compared to HFD group. ^#P < 0.05, ^##P < 0.01 compared to the Normal group