Fig. 8

Nrf2 regulates CaMKIIα transcription through binding to its promoter. Schematic diagram of Nrf2-binding site (antioxidant response elements, ARE) which locates in − 960 bp of the CaMKIIα promoter. a Nrf2 ChIP followed by PCR amplification of the putative CaMKIIα ARE. Following Nrf2 ChIP, qPCR was done to measure the presence of CaMKIIα ARE, NQO1 ARE (positive control), and RPL30 Exon 3 (negative control). b Representative western blotting images and the quantification and statistical analysis results showed the level of NQO1 and CaMKIIα with or without Nrf2 knockdown. c All results are presented as the mean ± SEM from three repeated experiments. The differences in the two groups were obtained using Student’s t-test. **P < 0.01, and ***P < 0.001