Fig. 4

The overexpression of Nrf2 triggers the activation of autophagy in ESCC cells. The autophagy levels of wild-type (WT) and Nrf2-overexpressed (Nrf2-OE) ESCC cells were detected by flow cytometry. Representative images (left) and quantitative data (right) showed autophagy in Ec109 and KYSE-30 cells by flow cytometry analysis. The differences in the two groups were obtained using Student’s t-test. *P < 0.05 and **P < 0.01