Fig. 3

TNF-α and IL-1β repress the adipogenesis of hADSCs. a hADSCs were cultured with adipogenesis-inducing medium (AD) with or without IL-6, IL-12, TNF-α and IL-1β (5 ng/mL each), respectively. Medium was changed every 3 days. After being cultured for 9 days, cells were fixed for triglycerides staining with Oil Red O. b Expression of adipogenesis-related genes in hADSCs treated with TNF-α/IL-1β (0.1 ng/mL each) was determined by Q-PCR assay on day 3. c Western blot analysis of PPAR-γ in adipogenic hADSCs interfered with TNF-α or IL-1β cytokines at different concentration. Scale bars, 100 μm