Fig. 4
From: MicroRNA let-7b inhibits cell proliferation via upregulation of p21 in hepatocellular carcinoma
P21 was predicted to be a targeted gene of let-7b. a Targeted gene analysis was performed by RNA22. The results showed that the 3′-UTR of the p21 gene contained binding sites for let-7b. b p21 expression was determined by luciferase reporter gene assay. c, d p21 expression was determined by RT-PCR when HepG2 and QGY-7703 cell lines were transfected with negative control (NC) and let-7b mimics (Let-7b). Data from three replicates are shown as the means (± SD). **P < 0.01, ***P < 0.001. E. p21 expression was determined by western blot when HepG2 and QGY-7703 cell lines were transfected with negative control (NC) and let-7b mimics (Let-7b)