Fig. 4

The effects of DOR activation on autophagy in SH-SY5Y cells after OGD/R injury. Western blot analysis of LC3 II/I (a) and P62 (b) levels in the OGD/R model (N = 6 per group). c Representative images of SH-SY5Y cells infected with Ad-mCherry-GFP-LC3B in each group. The yellow dots represent autophagosomes, and the red dots represent autolysosomes. Scale bar = 10 μm. d Statistical analysis of the percentages of red and yellow dots in cells (N = 5 per group). The values are shown as the mean ± SEM. Significant differences: the OGD/R group vs. the DADLE group (***p < 0.001) and the naltrindole group (p > 0.05, ns), the DADLE group vs. the DADLE + naltrindole group (p > 0.05, ns) in a; the OGD/R group vs. the DADLE group (*p < 0.05) and the naltrindole group (p > 0.05, ns), the DADLE group vs. the DADLE + naltrindole group (*p < 0.05) in b; the normal naltrindole group vs. the normal DADLE + naltrindole group (p > 0.05, ns), the OGD/R group vs. the DADLE group (**p < 0.01) and the naltrindole group (p > 0.05, ns), the DADLE group vs. the DADLE + naltrindole group (*p < 0.05) in d. DAD DADLE, Nal naltrindole