Three dimensional microcarrier system in mesenchymal stem cell culture: a systematic review

Koh, Benson; Sulaiman, Nadiah; Fauzi, Mh Busra; Law, Jia Xian; Ng, Min Hwei; Idrus, Ruszymah Bt Hj; Yazid, Muhammad Dain

doi:10.1186/s13578-020-00438-8

Cell & Bioscience

Table 2 Summary and classification of the microcarrier used in the 14 studies selected

From: Three dimensional microcarrier system in mesenchymal stem cell culture: a systematic review

Availability	Microcarrier	Material	Surface feature	Diameter (µm)	Porosity	Concentration	Cell yield	Cell differentiation	Manufacturer	Reference
Commercially available	Cell expansion
	Glass microcarrier (G102-1521)	Cross-linked polystyrene	Silica glass	125–212	Non-porous	35 mg/mL	–	–	Pall corporation	[56]
	Hillex II microcarrier	Polystyrene	Positive charge amine	160–200	Microporous	3 cm² per 24-well	1.2 × 10^5 cells/mL	–	Pall corporation	[58]
	Solohill plastic	Plastic	Plastic surface	90–150	Non-porous	6000 unit/mL	1–5 × 10^5 cells/mL	–	Pall corporation	[54]
						5 cm/mL	8.1 × 10^5 cells/mL^a	–		[61]
						5 cm/mL	9.6 × 10^4 cells/mL^b	–		[63]
	Solohill collagen	Polystyrene	Collagen coated	125–212	Non-porous	6000 unit/mL	1–5 × 10^5 cells/mL	–	Pall corporation	[54]
	Cytopores 1	Cellulose	Positive charge (0.9–1.20 meq/g)	230	Macroporous	1.2 mg/mL	1.5 × 10^5 cells/mL	–	GE Healthcare	[70]
	Cytopores 2	Cellulose	Positive charge (1.65–1.95 meq/g)	230	Macroporous	1.2 mg/mL	1.4 × 10^5 cells/mL	–	GE Healthcare	[70]
	Cell expansion & differentiation
	Cytodex 1	Cross-linked de × tran	Positive charge	147–247	Microporous	2.7 mg/mL	5.2 × 10^5 cells/mL^b	Chondrogenesis	GE Healthcare	[69]
						3 mg/mL	4.3 × 10^5 cells/mL	–		[70]
						1.7 mg.mL	0.86 × 10^5 cells/mL	–		[51]
	Cytodex 3	Cross-linked dextran	Gelatin coated	141–211	Microporous	4 mg/mL	3.55 × 10^5 cells/mL^b	Chondrogenesis	GE Healthcare	[69]
	Cytodex 3	Cross-linked dextran	Gelatin coated	141–211	Microporous	–	–	Osteogenesis		[57]
	CultiSpher S	Gelatine	Gelatine	130–380	Macroporous	0.5 mg/mL	2.46 × 10^5 cells/mL^b	Chondrogenesis	Sigma	[69]
						2 mg/mL	–	Vascularise bone-like microtissue		[65]
						1 mg/mL	1.3 × 10^5 cells/mL			[60]
	SphereCol	–	Collagen	125–212	Microporous	1.2 × 10^3 microcarrier/mL	3.58 × 10^4 cells/mL^b	Chondrogenesis	Sigma	[69]
	Silica glass microsphere	Silica	Silica	0.15–5	Non-porous	16 mg/mL	3.3 × 10^5 cells/mL	Osteogenesis	Polysciences Inc	[55]
In-house fabrication/modification	Cell expansion
	PVG coated microcarrier	Polystyrene	PVG-RGD coated	125–212	Non-porous	–	–	–	–	[59]
	PNIPAM grafted microcarrier	Polystyrene	PNIPAM coated	50–100		10 mg/mL	9.4 × 10^4 cells/mL	–	–	[62]
	Alginate-chitosan microcarrier	Genipin cross-linked-alginate	Chitosan	200–300		–	1.72 × 10^5 cells/mL	–	–	[51]
	PNIPAAm-grafted microcarrier	Cross-linked polystyrene	Thermosensitive PNIPAAm grafted surface	125-212	Non-porous	35 mg/mL	–	–	–	[56]
	PEG coated hydrogel	Polystyrene	PEG hydrogel surface	400	Non-porous	3 cm² per 24-well	1.12 × 10^5 cells/mL	–	–	[58]
	Cell expansion & differentiation
	Ti5 microcarrier	Titanium phosphate glass	Titanium phosphate glass	50–100	Non-porous	16 mg/mL	4.5 × 10^5 cells/mL	Osteogenesis	–	[55]
	Agarose microcarrier	Agarose	Casein	100–150		–	–	Osteogenesis	–	[57]

^aMicrocarrier added in mid of study
^bApproximated value

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ISSN: 2045-3701

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