Identification of hub genes associated with RNAi-induced silencing of XIAP through targeted proteomics approach in MCF7 cells

Gholizadeh, Mehdi Agha; Shamsabadi, Fatemeh T.; Yamchi, Ahad; Golalipour, Masoud; Jhingan, Gagan Deep; Shahbazi, Majid

doi:10.1186/s13578-020-00437-9

Table 2 Differentially expressed proteins in response to siRNA against XIAP in MCF-7 cells

From: Identification of hub genes associated with RNAi-induced silencing of XIAP through targeted proteomics approach in MCF7 cells

Spot	Gene product	Score	Uniprot ID	Biological process	Molecular function	Theoretical MW (KD)/pI	On gel MW (KD)/pI	Location	Ratio^*
1	GRP78	286	P11021	Protein metabolism	Chaperone activity	72,402/5.07	77,112/4.65	9q33	0.33
2	ENPL	82	P14625	Protein metabolism	Heat shock protein activity	92,696/4.76	80,516/4.55	12q23	0.27
3	ATPB	390	P06576	Metabolism, Energy pathways	Transporter activity	56,525/5.26	52,427/4.65	12q13	0.66
4	GDIB	103	P50395	Transport	Auxiliary transport protein activity	51,087/6.11	46,950/6.1	10p15	2.3
5	GRP75	274	P38646	Protein metabolism	Chaperone activity	73,920/5.87	71,587/5.9	5q31	0.51
6	G6PD	257	P11413	Metabolism, Energy pathways	Catalytic activity	59,675/6.39	55,639/6.1	Xq28	0.49
7	ENO1	735	P06733	Metabolism, Energy pathways	Catalytic activity	47,481/7.01	45,701/6.3	1p36	3.3
8	ALBU	73	P02768	Transport	Transporter activity	71,317/5.92	66,546/5.4	4q13	0.51
9	PRDX2	401	P32119	Metabolism; Energy pathways	Peroxidase activity	22,049/5.66	22,089/5.4	19p13	0.39
10	TCPE	122	P48643	Protein metabolism	Chaperone activity	60,089/5.45	64,545/5.3	5p15	0.64
11	FKBP4	102	Q02790	Metabolism, Energy pathways	Isomerase activity	52,057/5.35	50,535/5.2	12p13	0.43
12	HS71A	234	P0DMV8	Protein metabolism	Chaperone activity	70,294/5.48	71,325/5.3	6p21	0.68
13	HS71B	234	P0DMV9	Protein metabolism	Chaperone activity	70,294/5.48	81,261/5.3	6p21	0.41
14	HSP7C	526	P11142	Protein metabolism	Heat shock protein activity	71,082/5.37	71,261/5.4	11q24.1	0.68
15	HS90A	32	P07900	Protein metabolism	Chaperone activity	85,006/4.94	91,559/4.7	14q32	0.1
16	NPM	109	P06748	Protein metabolism	Chaperone activity	32,726/4.64	38,294/4.3	5q35	0.65
17	CRK	103	P46108	Cell communication, Signal transduction	Receptor signaling complex scaffold activity	338,675.38	29,195/5.0	17p13	0.03
18	PA2G4	70	Q9UQ80	Regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolism	Transcription regulator activity	44,101/6.13	70,963/6.3	12q13	0.63
19	SERA	140	O43175	Metabolism; Energy pathways	Catalytic activity	57,356/6.29	77,142/5.4	1p12	2.72
20	TBA1B	840	P68363	Cell growth and/or maintenance	Structural molecule activity	50,804/4.94	58,606/4.6	12q13	0.65
21	SAM50	36	Q9Y512	unknown	unknown	52,342/6.44	17,144/5.4	22q13	0.07
22	KPYM	118	P14618	Energy pathways, metabolism	Kinase activity	58,470/7.96	60,963/6.3	15q23	0.63
23	PRDX6	112	P30041	Metabolism, Energy pathways	Peroxidase activity	25,133/6.00	27,314/5.9	1q25	0.06
24	HSPB1	414	P04792	Protein metabolism	Chaperone activity	22,826/5.98	20,312/5.7	7q11	0.41
25	CH60	623	P10809	Protein metabolism	Heat shock protein activity	61,187/5.70	68,904/5.4	2q33	2.3
26	TCPZ	93	P40227	Protein metabolism	Chaperone activity	58,444/6.23	60,904/6.0	7p11	0.44
27	TBB5	504	P07437	Cell growth and/or maintenance	Structural constituent of cytoskeleton	50,095/4.78	54,486/4.8	6p21	0.75
28	IF5A1	90	P63241	Protein metabolism	Translation factor activity, nucleic acid binding	17,049/5.08	1646/4.8	17p13	0.6
29	EF1G	148	P26641	Protein metabolism	Translation regulator activity	50,429/6.25	53,625/5.9	11q12	0.5
30	PDIA1	635	P07237	Protein metabolism	Isomerase activity	57,480/4.76	60,784/4.5	17q25	0.83

The product of CH60, ENO1, and GDIB genes were increasingly expressed (> twofold)

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ISSN: 2045-3701

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