Fig. 2
SHP1 deficiency promotes the immunosuppressive effect of MSCs in vitro. Fresh C57BL/6 splenocytes were cocultured with mev/mev MSCs or WT MSCs and stimulated with anti-CD3 and anti-CD28 (1 μg/ml) each. Various MSC-to-splenocyte ratios were applied as indicated. a The extent of cell aggregation was observed by microscope after 48 h. Scale bar: 50 μm. b The area of cell clusters was quantitated using ImageJ software. c Proliferation was analyzed by 3H-Tdr incorporation after 48 h. The experiments were repeated at least three times. Error bars represent ± SEM (n = 3), ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001