Fig. 1

BMP2K is involved with normal and malignant megakaryopoiesis. a Public database (BloodSpot) shows upregulation of BMP2K in megakaryocytes. b Human CD34⁺ HSC were cultured under megakaryocyte differentiation medium for different days as indicated. The upregulation of BMP2K mRNA was confirmed by quantitative RT-PCR. c–d Upregulation of BMP2K mRNA in mononuclear cells isolated from AML (c) and AMKL (d) patients was confirmed by quantitative RT-PCR compared with that from peripheral blood of heathy (Normal, n = 7) donors. e–f CMK cells were induced to undergo megakaryocytic differentiation by MLN8237 (1 μM) treatment for 48 h, which was measured by staining the surface markers CD41 and CD61 (e) or staining the DNA with DAPI (f). Gates represent cells with diploidy (2 N), tetraploidy (4 N) and octaploidy (8 N). g Downregulation of BMP2K mRNA in CMK cells undergoing MLN8237-induced megakaryocytic differentiation for different hours was confirmed by quantitative RT-PCR. h The BMP2K protein in MLN8237-treated cells was also measured by immunoblotting. The right panel is the statistics of the densitometric analysis of the immunoblotting. * p < 0.05, ** p < 0.01; *** p < 0.001; NS, non-significance for the comparison as indicated