Fig. 7

P2X7 agonist BzATP attenuated the BTX-A-mediated promotion of microglial M2 polarization. a Western blot analysis of P2X7 protein level, b relative mRNA levels of iNOS and Arg-1 determined by qRT-PCR, and levels of M1-related TNF-α (c) and IL-6 (d) and M2-related IL-10 (e) in HAPI cells in the groups of Ctrl, LPS, LPS + BTX-A, and LPS + BTX-A + BzATP. BTX-A: 0.1 nM; LPS (100 ng/mL) was used as a microglial activator; BzATP (200 μM) was used as a P2X7 agonist. ^**P < 0.01, vs. Ctrl; ^##P < 0.01, vs. LPS; ^$P < 0.05, vs. LPS + BTX-A