Table 1 Summary of the interactions among replisome components, histones and histone chaperones
From: The replisome guides nucleosome assembly during DNA replication
Replisome proteins | Histone/histone chaperones | Methods used | Species |
|---|---|---|---|
PCNA | CAF-1 | IP: PCNA-CAF-1 physical interaction In vitro pull down: Cac1 (p150) subunit binds PCNA directly via the PIP domain | Homo sapiens, Saccharomyces cerevisiae |
RFC | Asf1 | In vitro pull down: Asf1 binds the Rfc1 subunit directly via the Asf1 N-terminal | Saccharomyces cerevisiae |
Rtt106 | IP: Rtt106-Elg1-RFC physical interaction | Saccharomyces cerevisiae | |
RPA | FACT | In vitro pull down: Pob3-M domain binds RPA directly | Saccharomyces cerevisiae |
H3–H4/FACT, CAF-1, Rtt106 | In vitro pull down: RPA binds H3-H4 directly. RPA binds FACT, CAF-1 and Rtt106 directly | Saccharomyces cerevisiae | |
Pol1 (Pol α) | H2A–H2B | IP: Yeast Pol1-(H2A–H2B) physical interaction In vitro pull down: N-terminal of human and yeast Pol1 has an H2A-H2B-binding domain | Saccharomyces cerevisiae, Homo sapiens |
FACT | IP: Pol α-FACT physical interaction In vitro pull down: Pol1 binds FACT directly | Saccharomyces cerevisiae | |
Dpb3-Dpb4 (Pol ε) | H2A–H2B | IP: Yeast Dpb3-Dpb4 has physical interaction with H2A-H2B and H3-H4 | Saccharomyces cerevisiae, Schizosaccharomyces pombe |
H3–H4 | Structural analysis and in vitro pull down: Dpb3-Dpb4 binds H3–H4 directly | Homo sapiens, Saccharomyces cerevisiae, Schizosaccharomyces pombe | |
Mcm2 (MCM2-7) | H3–H4 | IP: Mcm2-(H3–H4) physical interaction In vitro pull down: N-terminal terminus of Mcm2 has a (H3–H4)-binding domain | Mus musculus, Homo sapiens, Saccharomyces cerevisiae |
Asf1 | Structure analysis and in vitro pull down: H3–H4 dimer bridges the Asf1-Mcm2-7 interaction | Homo sapiens, Xenopus laevis | |
FACT | In vitro pull down: FACT binds Mcm2 directly | Homo sapiens, Saccharomyces cerevisiae |