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Table 1 Summary of the interactions among replisome components, histones and histone chaperones

From: The replisome guides nucleosome assembly during DNA replication

Replisome proteinsHistone/histone chaperonesMethods usedSpecies
PCNACAF-1IP: PCNA-CAF-1 physical interaction
In vitro pull down: Cac1 (p150) subunit binds PCNA directly via the PIP domain
Homo sapiens, Saccharomyces cerevisiae
RFCAsf1In vitro pull down: Asf1 binds the Rfc1 subunit directly via the Asf1 N-terminalSaccharomyces cerevisiae
Rtt106IP: Rtt106-Elg1-RFC physical interactionSaccharomyces cerevisiae
RPAFACTIn vitro pull down: Pob3-M domain binds RPA directlySaccharomyces cerevisiae
H3–H4/FACT, CAF-1, Rtt106In vitro pull down: RPA binds H3-H4 directly. RPA binds FACT, CAF-1 and Rtt106 directlySaccharomyces cerevisiae
Pol1 (Pol α)H2A–H2BIP: Yeast Pol1-(H2A–H2B) physical interaction
In vitro pull down: N-terminal of human and yeast Pol1 has an H2A-H2B-binding domain
Saccharomyces cerevisiae, Homo sapiens
FACTIP: Pol α-FACT physical interaction
In vitro pull down: Pol1 binds FACT directly
Saccharomyces cerevisiae
Dpb3-Dpb4 (Pol ε)H2A–H2BIP: Yeast Dpb3-Dpb4 has physical interaction with H2A-H2B and H3-H4Saccharomyces cerevisiae, Schizosaccharomyces pombe
H3–H4Structural analysis and in vitro pull down: Dpb3-Dpb4 binds H3–H4 directlyHomo sapiens, Saccharomyces cerevisiae, Schizosaccharomyces pombe
Mcm2 (MCM2-7)H3–H4IP: Mcm2-(H3–H4) physical interaction
In vitro pull down: N-terminal terminus of Mcm2 has a (H3–H4)-binding domain
Mus musculus, Homo sapiens, Saccharomyces cerevisiae
Asf1Structure analysis and in vitro pull down: H3–H4 dimer bridges the Asf1-Mcm2-7 interactionHomo sapiens, Xenopus laevis
FACTIn vitro pull down: FACT binds Mcm2 directlyHomo sapiens, Saccharomyces cerevisiae