Fig. 6

Functional analysis of the newly identified miR-0308 in both HepG2 and 7721 cell lines. a HepG2 cells were transfected with 50 nM of miR-0308 mimic or miR-NC and cell proliferation was evaluated via. A CCK-8 assay 48 h after transfection. *p < 0.05 as compared to control miR-NC treated cells. b The effects of miR-0308 on the distribution of G₁ and S phases in cells using flow cytometry. A representative flow cytometry histogram of the cell-cycle progression of HepG2 cells is shown with and without the miR-0308 mimic. c Quantitative measurement of the G₁ and S phases in HepG2 cells with or without the miR-0308 mimic. d The effect of miR-0308 on the distribution of G₁ and S phases in irradiated cells using flow cytometry. A representative flow cytometry histogram of cell cycle progression of 7721 cells is shown with or without the miR-0308 mimic. e Quantitative measurement of the G₁ and S phases of 7721 cells with or without the miR-0308 mimic. f Annexin V Flow cytometry assay of apoptosis in HepG2 cells co-transfected with or without the miR-0308 mimic. g Quantification of apoptosis, displaying the effects of miR-0303 on induction of apoptosis in HepG2 cells. h Annexin V Flow cytometry assay of apoptosis in 7721 cells co-transfected with or without the miR-0308 mimic. i Quantification of the apoptosis, displaying the effects of miR-0303 on the induction of apoptosis in 7721 cells. All data are presented as mean ± SD of three independent experiments; *p < 0.05 compared to the control group