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Fig. 6 | Cell & Bioscience

Fig. 6

From: SNHG6 modulates oxidized low-density lipoprotein-induced endothelial cells injury through miR-135a-5p/ROCK in atherosclerosis

Fig. 6

miR-135a-5p modulated ox-LDL-induced injury of HUVECs via targeting ROCK1 and ROCK2. ox-LDL-induced HUVECs were transfected with miR-NC mimics, miR-135a-5p mimics, miR-135a-5p mimics + Vector, miR-135a-5p mimics + ROCK1, or miR-135a-5p mimics + ROCK2. a, b The expression of ROCK1 or ROCK2 was examined by RT-qPCR. c Cell viability was measured by CCK-8 assay. d Cell apoptosis was detected by flow cytometry. e The protein expression of Bax and Bcl-2 was tested by western blot assay. f Caspase-3 activity was evaluated by using commercially-available kit. g, h The secretion of IL-6 and TNF-α were detected by ELISA assay. i, j The production of ROS and MDA were examined using commercially-available kits. **P < 0.01; #P < 0.05 and ##P < 0.01

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