Fig. 11
From: Enhanced efficiency in isolation and expansion of hAMSCs via dual enzyme digestion and micro-carrier
Porous microspheres cultured hAMSCs isolated from human amniotic membrane by Dual enzyme method morphology 1. CMs; 2. GCMs; 3. CCMs. a pre-treated microspheres; b 3 days of incubations; c 7 days of incubation. Cells were seeded at a density of 1 × 106. Live plus dead hAMSCs isolated from the dual enzyme digestion were counted in a haemocytometer. Scale bars a = 400 nm; b, c = 100 nm