Table 3 RT-qPCR primer sequences
From: CRISPRa-mediated FOXP3 gene upregulation in mammalian cells
Gene | Forward (5′−> 3′) | Reverse (5′−> 3′) | RefSeq accession number |
|---|---|---|---|
CD25 | cgcagaataaaaagcgggtca | acttgtttcgttgtgttccga | NM_000417.2 |
dCas9 | ggatcgaagagggcatcaaa | gttcctggtccacgtacatatc | KR011748.1 |
FOXP3 | gcaccttcccaaatcccagt | ggccacttgcagacacca | NM_014009.3 |
GAPDH | tgcaccaccaactgcttagc | ggcatggactgtggtcatgag | NM_002046.7 |
GITR | ccagtgtatcgactgtgcctcg | cacagcgttgtgggtcttgttc | NM_004195.3 |
ICOS | cccataggatgtgcagcctttg | ggctgtgttcactgctctcatg | NM_012092 |
IKZF2 | acactctggagagaagccgttc | ccagtgaactgcgctgcttgta | NM_016260.3 |
IRF4 | gaacgaggagaagagcatcttcc | cgatgccttctcggaactttcc | NM_002460.4 |
PI16 | ctggtgtgcaactatgagcctc | ggcaaatcctgagcatcttccg | NM_153370.3 |
PTPRC | cttcagtggtcccattgtggtg | ccactttgttctcggcttccag | NM_002838.5 |
TNFR2 | cgttctccaacacgacttcatcc | acgtgcagactgcatccatgct | NM_001066.3 |