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Table 3 RT-qPCR primer sequences

From: CRISPRa-mediated FOXP3 gene upregulation in mammalian cells

GeneForward (5′−> 3′)Reverse (5′−> 3′)RefSeq accession number
CD25cgcagaataaaaagcgggtcaacttgtttcgttgtgttccgaNM_000417.2
dCas9ggatcgaagagggcatcaaagttcctggtccacgtacatatcKR011748.1
FOXP3gcaccttcccaaatcccagtggccacttgcagacaccaNM_014009.3
GAPDHtgcaccaccaactgcttagcggcatggactgtggtcatgagNM_002046.7
GITRccagtgtatcgactgtgcctcgcacagcgttgtgggtcttgttcNM_004195.3
ICOScccataggatgtgcagcctttgggctgtgttcactgctctcatgNM_012092
IKZF2acactctggagagaagccgttcccagtgaactgcgctgcttgtaNM_016260.3
IRF4gaacgaggagaagagcatcttcccgatgccttctcggaactttccNM_002460.4
PI16ctggtgtgcaactatgagcctcggcaaatcctgagcatcttccgNM_153370.3
PTPRCcttcagtggtcccattgtggtgccactttgttctcggcttccagNM_002838.5
TNFR2cgttctccaacacgacttcatccacgtgcagactgcatccatgctNM_001066.3