Fig. 6
From: Hsa_circ_0051079 functions as an oncogene by regulating miR-26a-5p/TGF-β1 in osteosarcoma
Circ_0051079 promoted osteosarcoma cell proliferation and metastasis through miR-26a-5p/TGF-β1. a TGFB1 mRNA and protein levels in U2OS and SaoS2 cells. U2OS and SaoS2 cells were transfected with circ_0051079 plasmids and circ_0051079 levels were assayed by real time RT-PCR. b, c Cell proliferation in U2OS and SaoS2 cells. U2OS and SaoS2 cells were transfected with circ_0051079, miR-26a-5p mimics or TGF-β1 shRNAs for 24 h and then cell proliferation was assayed by MTT. d, e Cell migration in U2OS and SaoS2 cells. U2OS and SaoS2 cells were transfected with circ_0051079, miR-26a-5p mimics or TGF-β1 shRNAs for 24 h and then cell migration was assayed by transwell chamber. f, g Cell invasion in U2OS and SaoS2 cells. U2OS and SaoS2 cells were transfected with circ_0051079, miR-26a-5p mimics or TGF-1 shRNAs for 24 h and then cell invasion was assayed by transwell system. **p < 0.01