Fig. 4From: Customized one-step preparation of sgRNA transcription templates via overlapping PCR Using short primers and its application in vitro and in vivo gene editinga The electropherogram about the result of detection of fragment cleavage site in vitro. EGFR, pu57-1 and pu57-2 represent the PCR fragments with 664 bp, 727 bp and 727 bp length respectively. As the negative control “−” indicates that the gene is not digested by Cas9/sgRNA. “+” indicates that the result of digestion by Cas9/sgRNA. b–d The representative sequencing results of the cleaved fragments of EGFR, pu57-1 and pu57-2 respectivelyBack to article page