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Fig. 4 | Cell & Bioscience

Fig. 4

From: Customized one-step preparation of sgRNA transcription templates via overlapping PCR Using short primers and its application in vitro and in vivo gene editing

Fig. 4

a The electropherogram about the result of detection of fragment cleavage site in vitro. EGFR, pu57-1 and pu57-2 represent the PCR fragments with 664 bp, 727 bp and 727 bp length respectively. As the negative control “−” indicates that the gene is not digested by Cas9/sgRNA. “+” indicates that the result of digestion by Cas9/sgRNA. bd The representative sequencing results of the cleaved fragments of EGFR, pu57-1 and pu57-2 respectively

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