Fig. 4
![Fig. 4](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs13578-019-0350-7/MediaObjects/13578_2019_350_Fig4_HTML.png)
a The electropherogram about the result of detection of fragment cleavage site in vitro. EGFR, pu57-1 and pu57-2 represent the PCR fragments with 664 bp, 727 bp and 727 bp length respectively. As the negative control “−” indicates that the gene is not digested by Cas9/sgRNA. “+” indicates that the result of digestion by Cas9/sgRNA. b–d The representative sequencing results of the cleaved fragments of EGFR, pu57-1 and pu57-2 respectively