Fig. 2From: Customized one-step preparation of sgRNA transcription templates via overlapping PCR Using short primers and its application in vitro and in vivo gene editingA schematic diagram of overlapping primers amplification. AF1, AF2, AF3 and Tracr-R are overlapping primer. The sgRNA transcription template consists of three parts: T7 promotor (Red marked), the designed sequence of sgRNA (blue background marked), the conserved sequence of sgRNA (the rest of the sequence)Back to article page