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Fig. 2 | Cell & Bioscience

Fig. 2

From: Long noncoding RNA lnc-DILC stabilizes PTEN and suppresses clear cell renal cell carcinoma progression

Fig. 2

lnc-DILC reduces the proliferative ability of ccRCC cells. a lnc-DILC expression levels in the ccRCC cell lines and a normal human renal cell line (HK-2) were detected by qRT-PCR. b qRT-PCR assays for the lnc-DILC levels in ACHN and 786-O cells transfected with pcDNC3.1-lnc-DILC or the empty vector. c qRT-PCR assays for the lnc-DILC levels in Caki-1 cells transfected with two different siRNAs targeting lnc-DILC (si#1 and si#2). d The cell proliferation of ACHN and 786-O in response to lnc-DILC knockdown was measured using CCK-8 assay. e Colony formation assays performed with the ACHN and 786-O cells transfected with pcDNC3.1-lnc-DILC or the empty vector. f The cell proliferation of Caki-1 in response to lnc-DILC overexpression was measured using CCK-8 assay. g Colony formation assays performed with the Caki-1 cells transfected with lnc-DILC or negative control siRNAs. h Apoptosis was assayed by flow cytometry in ACHN and 786-O cells after overexpression of lnc-DILC. i Apoptosis was assayed by flow cytometry in Caki-1 cells after knockdown of lnc-DILC. j Caspase-3 activity of ACHN and 786-O cells was assayed after overexpression of lnc-DILC. k Caspase-3 activity of Caki-1 cells was assayed after knockdown of lnc-DILC. l The cell cycle analysis of ACHN and 786-O transfected with pcDNC3.1-lnc-DILC or the empty vector was performed using flow cytometry. The cell cycle distribution was listed with the percentage of G1, S, and G2 phases in the graphs. m The cell cycle analysis of Caki-1 cells transfected with lnc-DILC or negative control siRNAs was performed using flow cytometry. *p < 0.05, **p < 0.01, ***p < 0.001

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