Fig. 2

UCA1 promotes the expression of MMP9 in HTR-8 cells. a HTR-8 cells were used for the transfection with empty vector or UCA1 overexpression vector. The mRNA levels of the MMPs members were analyzed using quantitative RT-PCR 36 h post-transfection. *P < 0.05; UCA1-OE vs vector. b–d Levels of MMP2, MMP9, and TIMP1 mRNA was determined by quantitative RT-PCR in HTR-8 cells 36 h after transfection with siCtrl or siUCA1. *P < 0.05; siUCA1 vs siCtrl. e Levels of MMP9 and MMP2 expression were examined by western blotting analyses in HTR-8 cells 48 h after transfection with siCtrl, siUCA1, control vector, or UCA1 overexpression vector. f, g Supernatant collected from HTR-8 cells was used for the gelatin zymography assay 48 h after transfection with empty vector or UCA1 overexpression vector. The images of gelatin zymography were captured with a gel imaging system (Tanon 3500R). *P < 0.05; UCA1-OE vs vector. h, i Supernatant collected from HTR-8 cells was used for the gelatin zymography assay 48 h after transfection with control siRNA or siUCA1. The images of gelatin zymography were captured with a gel imaging system (Tanon 3500R). *P < 0.05; siUCA1 vs siCtrl