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Fig. 1 | Cell & Bioscience

Fig. 1

From: Urothelial carcinoma associated 1 promotes trophoblast invasion by regulating MMP9

Fig. 1

UCA1 regulates trophoblast invasive and proliferative ability in vitro. a, b Quantitative RT-PCR analysis was utilized for the analysis of UCA1 mRNA expression in HTR-8 cells 48 h after transfection with control siRNA, siUCA1#1, siUCA1#2, siUCA1#3, empty vector, or UCA1-overexpression (OE) plasmid, respectively. *P < 0.05; siRNA vs siCtrl or UCA1-OE vs vector. c HTR-8 cells were used for the transfection with control siRNA, siUCA1 #3, empty vector, or UCA1-overexpression vector, respectively. Cell proliferation rate was evaluated by the CCK-8 assay at indicated several time points. *P < 0.05; siRNA vs siCtrl or UCA1-OE vs vector. d HTR-8 cells were used for the transfection with siCtrl, siUCA1#3, empty vector or UCA1 overexpression plasmid, respectively. Then the cells were cultured for 24 h to allow them to reach approximately 85% confluence, followed by the analysis for would healing abilities. Overexpression of UCA1 in HTR-8 cells led to the enhanced wound closure ability than that in the ones transfected with empty vector. UCA1-knockdown significantly decreased the extension of wound closure than that in the siCtrl group. Scale bars: 100 μm. e, f UCA1 overexpression markedly promoted cell invasion of either HTR-8 cells or freshly isolated primary trophoblast cells than that of control ones. Knockdown of UCA1 inhibited cell invasion than that of the siCtrl ones. Scale bars: 100 μm

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